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KMID : 0603820090150040355
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Journal of Experimental & Biomedical Science
2009 Volume.15 No. 4 p.355 ~ p.362
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The Effect of NMDA/glycine Receptor Antagonist, 7-Chlorokynurenic Acid on Cultured Astrocytes Damaged by Ischemia-like Condition
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Jung In-Ju
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Abstract
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I evaluated the protective effect of N-methyl-D-aspartate (NMDA)/glycine receptor antagonist, 7-chlorokinurenic acid (CKA) on cultured mouse astrocytes damaged by ischemia-like condition (ILC). The protective effect of CKA was assessed by cell viability, lactate dehydrogenase (LDH) activity, superoxide dismutase (SOD)-like activity and lipid peroxidation. To examine the effect of CKA on the cell apoptosis, the expression and the activity of caspase 3 were assessed by Western blotting. CKA increased the cell viability decreased by ILC. CKA also decreased the LDH activity and antioxidative effects such as SOD-like activity and inhibitory activity of lipid peroxidation. In addition, CKA suppressed the expression of caspase 3 associated with apoptosis, and increased the cell viability by the decrease of caspase 3 activity as like the caspase 3 inhibitor, Av-DVED-MED. From these results, these results suggest that ILS induces cell cytotoxicity in cultured astrocytes and CKA, NMDA/glycine receptor antagonist, is effective on the prevention of the cytotoxicity due to ILS by the antioxidative effect and the inhibition of apoptosis.
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KEYWORD
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7-chlorokinurenic acid, Apoptosis, NMDA/glycine receptor, Caspase 3
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